Analysis Note

ControlHuman cardiac muscle tissue

Application

Western Blot Analysis: 0.5 µg/mL from a representative lot detected Myosin-2 in L6 cell lysate (Myosin may be observed as a smear in some cell lysates).

Western Blot Analysis: A representative lot detected Myosin-2 in mouse heart cell cytosolic extracts.

This Anti-Myosin 2 Antibody, clone 5B11.1 is validated for use in Western Blotting, IHC for the detection of Myosin 2.

Research Sub CategoryCytoskeleton

Research CategoryCell Structure

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Myosin is a hexameric protein consisting of two heavy chains including Myosin-2 (MYH2), two regulatory light chains, and two alkali light chains. The myosin-2 heavy chain interacts with the actin cytoskeleton and mediates muscle contractility in eukaryotic cells. Defects in myosin-2 result in inclusion body myopathy type 3.

Immunogen

KLH-conjugated linear peptide corresponding to mouse Myosin 2.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified by Ion-Exchange Chromatography

Purified mouse monoclonal IgG3κ in buffer containing PBS with 0.05% sodium azide.

Format: Purified

Quality

Evaluated by Immunohistochemistry in human cardiac muscle tissue.

Immunohistochemistry Analysis: A 1:300 dilution of this antibody detected Myosin-2 in human cardiac muscle tissue.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~220 kDa observed